
Kraven The Hunter (Sergei Kravinoff) (First and only known appearance) (Not In 3DS Storyline).Dennis Carradine (Death) (Full name revealed).Black Cat (Felicia Hardy) (Last appearance).Shocker (Herman Schultz) (First and only known appearance).
Green Goblin (Harry Osborn) (First and only known appearance). Electro (Max Dillon) (First and only known appearance) (Apparent death). Chameleon (Dmitri Smerdyakov) (First and only known appearance) (Impersonates Donald Menken). Kingpin (Wilson Fisk) (First and only known appearance). Carnage Suit (First and only known appearance). Carnage (Cletus Kasady) (First and only known appearance). Aunt May (First and only known appearance).
Spider-Man (Peter Parker) (Last appearance) (Main story and flashback).